The Jamaican sprint King will captain the ‘Rest of the World’ XI against the ‘England’ team lead by pop star Robbie Williams. The match kicks off at Old Trafford on June 10.
Bolt, who announced his participation in the charity match on social media, teased that he signed for South African football club Sundowns on Monday.
"It is my dream to make it as a professional footballer, so to be able to step out onto the pitch at Old Trafford in June, and play against some of football’s biggest legends is going to be remarkable," Bolt told Manchester United's official website.
"I enjoy the thrill of competition in front of a crowd, so Robbie and his England team better watch out as I won’t be going easy on them!
"I’ve got a pretty special celebration planned for when I score, by the way. My team is going to be unbeatable – and Soccer Aid for Unicef is counting on your support to make a difference to thousands of children around the world. Come and join in the fun!"
The Olympian appeared in a photo holding a Sundowns shirt with his name on the back and wrote "I've signed for a football team!" on Twitter ahead of the announcement.
The Unicef supported initiative - which has raised over $30million in the last decade - raises money for underprivileged children across the world, providing them with food, vaccines and clean water.
Previous editions of the charity match have featured the likes of Hollywood stars Will Ferrell and Woody Harrelson, as well as former greats Zinedine Zidane and Diego Maradona.
